op9 mouse preadipocyte cell line Search Results


op9 preadipocytes  (ATCC)
96
ATCC op9 preadipocytes
Figure 2 The effect of sPLA2-IIE on lipid accumulation in <t>OP9</t> and 3T3-L1 cells. (A) sPLA2-IIE mRNA copies in OP9 cells before (Day 0) and after differentiation (Day 3). mRNA copies are expressed relative to Day 0 set at 100%. (B, C) Lipid droplets and triglyceride (TG) accumulation in the differentiated OP9 cells transfected with pcDNA4/sPLA2- IIE (OP9-pcDNA4/IIE) and si-sPLA2-IIE (OP9-si-IIE). Lipid droplets were detected using Oil Red O staining. TG was quantified at Day 3 of differentiation. (D, E) sPLA2-IIE protein levels in OP9 cells transfected with pcDNA4/sPLA2-IIE (OP9-pcDNA4/IIE) and si-sPLA2-IIE (OP9-si-IIE). Protein levels are expressed relative to the OP9 cells set at 100%. The data are expressed as the mean 6 SE. n 5 4 in each group. *P < 0.05 compared with the OP9 cells. (B, E) (F, G) Lipid droplets and TG levels in the differentiated 3T3-L1 cells treated with and without sPLA2-IIE protein. 3T3-L1 cells were incubated with PLA2-IIE protein for 8 days. The data are expressed as the mean 6 SE. n 5 4 in each group. *P < 0.05 compared with the corresponding control. [Color figure can be viewed in the online issue, which is available at wileyonlinelibrary.com.]
Op9 Preadipocytes, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/op9+mouse+preadipocyte+cell+line/OP9/pm25755141-62-1-12
Average 96 stars, based on 1 article reviews
op9 preadipocytes - by Bioz Stars, 2026-10
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primary-culture preadipocytes  (JCRB Cell Bank)
90
JCRB Cell Bank primary-culture preadipocytes
Figure 2 The effect of sPLA2-IIE on lipid accumulation in <t>OP9</t> and 3T3-L1 cells. (A) sPLA2-IIE mRNA copies in OP9 cells before (Day 0) and after differentiation (Day 3). mRNA copies are expressed relative to Day 0 set at 100%. (B, C) Lipid droplets and triglyceride (TG) accumulation in the differentiated OP9 cells transfected with pcDNA4/sPLA2- IIE (OP9-pcDNA4/IIE) and si-sPLA2-IIE (OP9-si-IIE). Lipid droplets were detected using Oil Red O staining. TG was quantified at Day 3 of differentiation. (D, E) sPLA2-IIE protein levels in OP9 cells transfected with pcDNA4/sPLA2-IIE (OP9-pcDNA4/IIE) and si-sPLA2-IIE (OP9-si-IIE). Protein levels are expressed relative to the OP9 cells set at 100%. The data are expressed as the mean 6 SE. n 5 4 in each group. *P < 0.05 compared with the OP9 cells. (B, E) (F, G) Lipid droplets and TG levels in the differentiated 3T3-L1 cells treated with and without sPLA2-IIE protein. 3T3-L1 cells were incubated with PLA2-IIE protein for 8 days. The data are expressed as the mean 6 SE. n 5 4 in each group. *P < 0.05 compared with the corresponding control. [Color figure can be viewed in the online issue, which is available at wileyonlinelibrary.com.]
Primary Culture Preadipocytes, supplied by JCRB Cell Bank, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/op9+mouse+preadipocyte+cell+line/primary+culture+preadipocytes/us10113147-647-3-22
Average 90 stars, based on 1 article reviews
primary-culture preadipocytes - by Bioz Stars, 2026-10
90/100 stars
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Image Search Results


Figure 2 The effect of sPLA2-IIE on lipid accumulation in OP9 and 3T3-L1 cells. (A) sPLA2-IIE mRNA copies in OP9 cells before (Day 0) and after differentiation (Day 3). mRNA copies are expressed relative to Day 0 set at 100%. (B, C) Lipid droplets and triglyceride (TG) accumulation in the differentiated OP9 cells transfected with pcDNA4/sPLA2- IIE (OP9-pcDNA4/IIE) and si-sPLA2-IIE (OP9-si-IIE). Lipid droplets were detected using Oil Red O staining. TG was quantified at Day 3 of differentiation. (D, E) sPLA2-IIE protein levels in OP9 cells transfected with pcDNA4/sPLA2-IIE (OP9-pcDNA4/IIE) and si-sPLA2-IIE (OP9-si-IIE). Protein levels are expressed relative to the OP9 cells set at 100%. The data are expressed as the mean 6 SE. n 5 4 in each group. *P < 0.05 compared with the OP9 cells. (B, E) (F, G) Lipid droplets and TG levels in the differentiated 3T3-L1 cells treated with and without sPLA2-IIE protein. 3T3-L1 cells were incubated with PLA2-IIE protein for 8 days. The data are expressed as the mean 6 SE. n 5 4 in each group. *P < 0.05 compared with the corresponding control. [Color figure can be viewed in the online issue, which is available at wileyonlinelibrary.com.]

Journal: Obesity (Silver Spring, Md.)

Article Title: Group IIE secretory phospholipase A2 regulates lipolysis in adipocytes.

doi: 10.1002/oby.21015

Figure Lengend Snippet: Figure 2 The effect of sPLA2-IIE on lipid accumulation in OP9 and 3T3-L1 cells. (A) sPLA2-IIE mRNA copies in OP9 cells before (Day 0) and after differentiation (Day 3). mRNA copies are expressed relative to Day 0 set at 100%. (B, C) Lipid droplets and triglyceride (TG) accumulation in the differentiated OP9 cells transfected with pcDNA4/sPLA2- IIE (OP9-pcDNA4/IIE) and si-sPLA2-IIE (OP9-si-IIE). Lipid droplets were detected using Oil Red O staining. TG was quantified at Day 3 of differentiation. (D, E) sPLA2-IIE protein levels in OP9 cells transfected with pcDNA4/sPLA2-IIE (OP9-pcDNA4/IIE) and si-sPLA2-IIE (OP9-si-IIE). Protein levels are expressed relative to the OP9 cells set at 100%. The data are expressed as the mean 6 SE. n 5 4 in each group. *P < 0.05 compared with the OP9 cells. (B, E) (F, G) Lipid droplets and TG levels in the differentiated 3T3-L1 cells treated with and without sPLA2-IIE protein. 3T3-L1 cells were incubated with PLA2-IIE protein for 8 days. The data are expressed as the mean 6 SE. n 5 4 in each group. *P < 0.05 compared with the corresponding control. [Color figure can be viewed in the online issue, which is available at wileyonlinelibrary.com.]

Article Snippet: The OP9 preadipocytes and 3T3-L1 mouse embryo preadipocytes were obtained from the American Type Culture Collection and were cultured following the procedure described elsewhere (18).

Techniques: Transfection, Staining, Incubation, Control

Figure 3 sPLA2-IIE regulates lipolysis rather than TG synthesis in adipocytes. (A, B) Free glycerol levels in the dif- ferentiated OP9, OP9-pcDNA4/IIE, OP9-si-IIE, differentiated 3T3-L1, 3T3-L11IIE, and the positive-control (TNF-a) groups. Levels are expressed relative to respective controls set at 100%. (C) mRNA levels of adipogenic genes in OP9, OP9-pcDNA4/IIE, and OP9-si-IIE cells before (Day 0) and after differentiation (Day 3). Levels are expressed relative to Day 0 set at 100%. The values are the mean 6 SE. n 5 4 in each experimental group. *P < 0.05 as compared with OP9 or 3T3-L1 cells.

Journal: Obesity (Silver Spring, Md.)

Article Title: Group IIE secretory phospholipase A2 regulates lipolysis in adipocytes.

doi: 10.1002/oby.21015

Figure Lengend Snippet: Figure 3 sPLA2-IIE regulates lipolysis rather than TG synthesis in adipocytes. (A, B) Free glycerol levels in the dif- ferentiated OP9, OP9-pcDNA4/IIE, OP9-si-IIE, differentiated 3T3-L1, 3T3-L11IIE, and the positive-control (TNF-a) groups. Levels are expressed relative to respective controls set at 100%. (C) mRNA levels of adipogenic genes in OP9, OP9-pcDNA4/IIE, and OP9-si-IIE cells before (Day 0) and after differentiation (Day 3). Levels are expressed relative to Day 0 set at 100%. The values are the mean 6 SE. n 5 4 in each experimental group. *P < 0.05 as compared with OP9 or 3T3-L1 cells.

Article Snippet: The OP9 preadipocytes and 3T3-L1 mouse embryo preadipocytes were obtained from the American Type Culture Collection and were cultured following the procedure described elsewhere (18).

Techniques: Positive Control

Figure 4 sPLA2-IIE-stimulated lipolysis is possibly mediated through the ERK pathway. (A) The levels of phospho- rylated extracellular signal-regulated kinases 1 and 2 (p-ERK1/2) and phosphorylated hormone-sensitive lipase (p-HSL) in the adipose tissue of sPLA2-IIE2/2 mice and WT mice. (B) p-ERK 1/2 levels in differentiated 3T3-L1 cells treated with sPLA2-IIE protein. (C) p-ERK1/2 and p-HSL levels in OP9, OP9-si-IIE, OP9-pcDNA4/IIE cells, and OP9-pcDNA4/IIE cells treated with PD98059. (D, E) Lipid droplets and free glycerol levels in OP9-si-IIE cells, OP9-pcDNA4/IIE cells, and OP9-pcDNA4/IIE cells treated with PD98059. Free glycerol levels are expressed rela- tive to OP9 cells set at 100%. Lipid droplets were detected using Oil Red O staining. n 5 4 in each experimental group. *P < 0.05 compared with OP9 cells; **P < 0.05 compared with OP9-pcDNA4/IIE group. [Color figure can be viewed in the online issue, which is available at wileyonlinelibrary.com.]

Journal: Obesity (Silver Spring, Md.)

Article Title: Group IIE secretory phospholipase A2 regulates lipolysis in adipocytes.

doi: 10.1002/oby.21015

Figure Lengend Snippet: Figure 4 sPLA2-IIE-stimulated lipolysis is possibly mediated through the ERK pathway. (A) The levels of phospho- rylated extracellular signal-regulated kinases 1 and 2 (p-ERK1/2) and phosphorylated hormone-sensitive lipase (p-HSL) in the adipose tissue of sPLA2-IIE2/2 mice and WT mice. (B) p-ERK 1/2 levels in differentiated 3T3-L1 cells treated with sPLA2-IIE protein. (C) p-ERK1/2 and p-HSL levels in OP9, OP9-si-IIE, OP9-pcDNA4/IIE cells, and OP9-pcDNA4/IIE cells treated with PD98059. (D, E) Lipid droplets and free glycerol levels in OP9-si-IIE cells, OP9-pcDNA4/IIE cells, and OP9-pcDNA4/IIE cells treated with PD98059. Free glycerol levels are expressed rela- tive to OP9 cells set at 100%. Lipid droplets were detected using Oil Red O staining. n 5 4 in each experimental group. *P < 0.05 compared with OP9 cells; **P < 0.05 compared with OP9-pcDNA4/IIE group. [Color figure can be viewed in the online issue, which is available at wileyonlinelibrary.com.]

Article Snippet: The OP9 preadipocytes and 3T3-L1 mouse embryo preadipocytes were obtained from the American Type Culture Collection and were cultured following the procedure described elsewhere (18).

Techniques: Staining